plzf d-9 mouse igg1 a680 antibody  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: Differential retinoic acid responses across testicular development in vitro

doi: 10.64898/2025.12.30.696417

Figure Lengend Snippet: CIVMs derived from neonatal testis from PND 5 and PND 10 testis were exposure to isotretinoin for 24 hours. (A) Expression of Stra8 in PND 5 derived CIVMs determined by RT-qPCR. In the control, 0.3, 3, and 30 nM isotretinoin dose groups the expression of Stra8 contained samples below the threshold of quantification. The expression of Stra8 was significantly increased in the 300 nM isotretinoin dose group (p-value <0.0001). (B) Expression of Plzf in PND 5 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 300 nM isotretinoin dose group was significantly lower than control (p-value of 0.026). (C) Expression of Stra8 in PND 10 derived CIVMs determined by RT-qPCR. Aside from one control sample, all samples were above the threshold of quantification. The expression of Stra8 was significantly increased in the 3,30, and 300 nM isotretinoin dose groups (p-values all <0.0001). (D) Expression of Plzf in PND 10 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 30 and 300nM isotretinoin dose groups were significantly lower than control (p-values <0.0001).

Article Snippet: RT-qPCR was used to target the rat genes Stra8 (Rn01747849_m1) and Plzf (Rn01418644_m1).

Techniques: Derivative Assay, Expressing, Quantitative RT-PCR, Control

Journal: bioRxiv

Article Title: Differential retinoic acid responses across testicular development in vitro

doi: 10.64898/2025.12.30.696417

Figure Lengend Snippet: The expression of Plzf and Stra8 quantified by RT-qPCR at day in vitro 4 and day in vitro 15 in PND 5 tissue CIVMs and PND 10 tissue CIVMs in control and media supplementation conditions. Color indicates day in vitro , dashed line divides plot by PND 5 CIVMs and PND 10 CIVMs. Points that are “X” indicate samples that were below the limit of quantification (cycle threshold of 37). Data displayed as expression relative to beta-actin (housekeeping gene (HK), (2^ [gene – HK])).

Article Snippet: RT-qPCR was used to target the rat genes Stra8 (Rn01747849_m1) and Plzf (Rn01418644_m1).

Techniques: Expressing, Quantitative RT-PCR, In Vitro, Control

Journal: Biology

Article Title: Construction and Performance Evaluation of an Astaxanthin–Chitosan/Chitooligosaccharide Hydrogel System for Ex Vivo Culture of Murine Spermatogonial Stem Cells

doi: 10.3390/biology14121664

Figure Lengend Snippet: Immunofluorescence profiling of molecular markers in mouse SSCs after 7 days on AST-CO/COS hydrogels. ( A , B ) OCT4 and MYC lines, immunofluorescence co-staining (white scale bar = 200 μm, in the lower-left corner of the image), and quantitative analysis of mean fluorescence intensity for stemness transcription factors OCT4 and MYC in CHAG, COAG, and CG groups (clone number = 20). ( C , D ) VASA and PLZF lines, immunofluorescence co-staining (white scale bar = 200 μm, in the lower-left corner of the image), and quantitative analysis of mean fluorescence intensity for SSC-specific markers VASA and PLZF in CHAG, COAG, and CG groups (clone number = 20, ** p < 0.01, *** p < 0.001, **** p < 0.0001). ( E , F ) CASP3 and P16 lines, immunofluorescence co-staining (white scale bar = 200 μm, in the lower-left corner of the image) and quantitative analysis of mean fluorescence intensity for senescence-related genes Caspase-3 and P16 in CHAG, COAG, and CG groups (clone number = 20, ** p < 0.01).

Article Snippet: The samples were then incubated with primary antibodies (OCT4, c-MYC, PLZF, VASA, PCNA, Ki67, Caspase-3, P16; Boster, Beijing, China, cat. MA00174, BM0238, PB0199, A02448, BM1592, PB9026, M00125-3, and PB9188, dilution 1:200) for 45 min at 37 °C, followed by corresponding Alexa Fluor 488- or 555-conjugated secondary antibodies (Boster, Beijing, China, cat. BA1127 and BA1141, dilution 1:500) for 30 min at 37 °C.

Techniques: Immunofluorescence, Staining, Fluorescence